Transforming bacteria with plasmid DNA¶
Contents:
- Background
- A. Make Luria broth (LB) and LB agar plates
- B. Transfer E.coli cells from glycerol stock to LB agar plates
- C. Grow E.coli cells
- D. Make competent E.coli cells
- E. Transform Competent E.coli cells
- F. Screen potentially transformed bacteria
- Day 4
- F.1 Growing potentially transformed bacteria
- Day 5
- F.2 Extract the plasmid DNA using the alkaline lysis method
- F.3 Visualize the plasmid DNA using agarose gel electrophoresis
- F.3.1 Prepare a 1% agarose gel
- F.3.2 Load the samples and run the gel
- F.3.3 Visualize the DNA fragments under ultraviolet (UV) light